FastQCFastQC Report
Thu 28 Apr 2016
ERR1042470.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042470.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6133410
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC389570.6351605387541351No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG320310.5222380372419259No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA233810.3812071914318462No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG195560.3188438405389498No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT174600.28467035466404494No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC153160.24971426987597437No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT147850.24105676939907816No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT133810.2181657511889797No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC87150.1420906151716582No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT86680.1413243204025167No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG83190.13563417413804066No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG82470.134460275768292No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68080.11099861251734353No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT67440.10995514729978918No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA64050.104428042475556No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT63970.10429760932336171No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG18650.018.053625
GACGGAC19100.016.9502627
CGGACCA19400.016.8788669
ACGGACC19300.016.7746128
TTAACGG5300.016.75471735
CGCAAGA19700.016.6218262
GCGCAAG19950.016.4135341
ACGAACG7550.015.92715315
TAAACGC16400.015.79268428
CGAACGA7700.015.61688216
TATAACG2150.015.4883722
TCTAGCG11000.015.47272828
CAAGACG22800.015.3355274
AGACGGA21400.015.3014036
CGCTTCG17600.015.0312532
CTAGCGG11450.015.02620129
TTCGGGC16750.015.02089535
GTAAACG17300.014.97109827
GCTTCGG18100.014.82044233
TAACGGC6000.014.836
Produced by FastQC (version 0.11.5)