FastQCFastQC Report
Thu 28 Apr 2016
ERR1042469.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042469.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1491318
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT111340.7465879175333496No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT95330.6392332151828115No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47320.31730321769066017No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT42280.2835076087058562No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC22500.15087325439644664No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA21940.1471181867314684No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT20970.14061387309748824No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG16930.11352374208585961No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACACT1700.019.5882344
AAGACGG5800.019.137935
CAACCGT500.00703583918.56
GCGCAAG5800.018.51
CGAACGT1800.018.4999984
CAAGACG6000.018.1916684
CGCAAGA5650.018.008852
TCGAACG1850.018.03
GACGGAC5700.017.5263167
ACGGACC5900.017.2457628
GCAAGAC7700.017.0584413
GCGGTAC1005.880147E-616.65000237
CGGACCA6650.015.3007529
TCGTTTA1701.4861143E-915.23529330
CTAACAC855.3654815E-415.2352933
GCGAAAG6500.015.08461518
ATAACGC2700.015.0740753
GGTTATC2950.015.0508472
TAATACC1853.0559022E-1015.04
CCGATAA2350.014.9574479
Produced by FastQC (version 0.11.5)