FastQCFastQC Report
Thu 28 Apr 2016
ERR1042468.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042468.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4211853
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT181850.4317577085430095No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT157700.37441952508788884No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT148070.35155547926292774No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC140320.33315502701542526No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG95780.2274058472601014No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA85860.2038532683832983No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74260.17631194630961716No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG55030.13065508221678201No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC50240.11928241560187404No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG49050.11645705583741882No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT46930.1114236418032633No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT43470.10320873021921705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG4100.022.1097552
TAACGCA4800.020.0416664
GTATAAC6550.016.381681
ATAACGC10750.016.004653
TTAGAGT9300.015.7150544
CAAGACG13500.015.4851864
AACCGTA1201.938386E-615.4166667
GACGGAC12700.014.8582697
CGCAAGA13100.014.8282442
TTAACGG2006.184564E-1114.835
AAGACGG13600.014.6911765
ACGGACC13250.014.6603788
ATTAGAG11700.014.230773
TAATACT8500.014.1470594
TAGAGTG10500.014.0952385
TATCACG4600.014.0760872
CTAGTAC2009.858923E-1013.8750013
TATAGCG800.006302297613.8755
CGAACGA4900.013.59183616
CGGTCCA10550.013.5023710
Produced by FastQC (version 0.11.5)