FastQCFastQC Report
Thu 28 Apr 2016
ERR1042467.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042467.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1833006
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT124170.677411857898992No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT105420.5751208670348051No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT51230.2794862646385227No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51070.2786133815164817No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC35400.1931253907515851No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA33590.18325090043349557No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT26070.14222539369756565No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG25360.13835197484350842No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG23940.13060513713539398No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG21990.11996687408551854No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT18680.10190910449829405No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAT358.871417E-426.428571
CGAACTA1002.877241E-718.524
TAGGTCG609.2396763E-418.55
AAGACGG6600.017.9393945
TATACTG3400.017.4117645
CGCAAGA6800.016.8676452
GCGAACT1107.813833E-716.81818223
TTAGAGT4400.016.8181824
CAAGACG7050.016.7943254
GCGCAAG6950.016.237411
TAGAGTG4800.016.18755
ACTAGAC1606.2937033E-1016.18753
GAACCGT803.383938E-416.18756
GACGGAC7250.015.5655167
ACGGACC7250.015.3103448
ATTAGAG5150.015.0873793
ACCGTCG4950.014.9494958
TACCCCG1001.0939273E-414.85
TCGAACG2251.8189894E-1214.83
TATAACG1904.5656634E-1014.6052642
Produced by FastQC (version 0.11.5)