FastQCFastQC Report
Thu 28 Apr 2016
ERR1042466.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042466.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9605407
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT295090.30721238569068443No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT233830.24343580652022345No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT194870.20287531803701808No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT146180.1521851182360102No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111650.11623661548125967No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT107520.11193695384276792No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCC99700.1037957058977303No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGGC5750.019.62608536
TTAACGG6150.018.65040635
AAGACGG36500.017.4863015
CAAGACG39350.016.7369754
ACGGACC37900.016.4010568
CGCAAGA38850.016.2380942
GACGGAC37900.016.2058057
TATAACG5000.015.912
TAACGCA5250.015.8571434
CGGACCA40050.015.7515619
GCGCAAG41250.015.696971
AGACGGA41550.015.0938636
CGAACGA17300.015.07803416
ACGAACG18250.014.4958915
ATAACGC24150.014.4782613
ATAACGA18650.014.38337812
TAATACT21600.014.2175934
GTATTAG35100.014.072651
TAACGAA19100.013.94764313
GCAAGAC52200.013.7863983
Produced by FastQC (version 0.11.5)