FastQCFastQC Report
Thu 28 Apr 2016
ERR1042465.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042465.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7622259
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1334851.7512524830237335No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1258401.65095413315134No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1191431.5630930410525279No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1045901.3721653908637845No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG999091.3107531507391708No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA830981.0902017367817074No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG648940.8513749008004057No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT475490.6238176897426341No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT459140.6023673559242739No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG443800.5822420885986687No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC389220.5106360201089992No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG388500.5096914182527779No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC317980.41717291422398534No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT307350.4032269173744949No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA290280.3808319816999134No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA232750.30535566949378135No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC221850.2910554469482079No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG213930.28066482652977287No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT203650.26717801113816786No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA182960.2400338272420289No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC174740.229249622716835No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG172180.22589103833915905No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162140.21271909023296115No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT161520.2119056830789927No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG161360.21169577155538796No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC158390.20779928889847485No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC156650.2055165010792732No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC153680.2016200184223601No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG142360.18676877812732418No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT136750.17940875533093273No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT133480.1751186885672607No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT129920.17044815716705505No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT124910.1638753025841814No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC119970.15739428429288482No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119830.15721061170973066No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG118650.15566251422314564No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117020.1535240405764223No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT116340.15263191660110212No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC114500.1502179340796475No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT113880.14940452692567913No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC112560.14767275685593995No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA110860.14544244691763952No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT109680.1438943494310545No Hit
GTACATGGGGGGTTGTGATCACTTCGCCCTCAGCTGTGTTGTG109100.14313342015798727No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA107020.14040457035112555No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT104800.1374920479611097No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT104250.13677047709871837No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT100750.13217866251986451No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA99640.13072240132485657No Hit
CCACAACACAGCTGAGGGCGAAGTGATCACAACCCCCCATGTA94750.12430698038468647No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG93910.12320494488576156No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA85530.11221082883696291No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82740.1085504966441051No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA77420.10157093848424727No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACAC48400.016.8181823
ATTAGAG10000.016.653
GTACTCC38750.016.2836
CCACAAC51600.016.0261611
TTAACGG5200.016.00961535
CGTTATA2700.015.7592592
TAACGGC5350.015.56074736
CGCCACA19650.014.781171
ACGGACC9300.014.7204298
TACCTGT45700.014.61378737
AAGACGG10450.014.51674655
CTAGCGG6550.014.4045829
CACAACA56650.014.4015882
AAAAGTA27050.014.29392
TGTACCT42850.014.29054835
GTATTAG16350.014.2568821
TCTAGCG6500.014.23076928
GTACTAG2600.014.2307681
CACGCAG8350.014.1796415
TGCGACG1702.4430847E-814.14705922
Produced by FastQC (version 0.11.5)