FastQCFastQC Report
Thu 28 Apr 2016
ERR1042464.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042464.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8926252
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC543850.6092702737946453No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT477230.5346364857277164No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG402950.4514212684114228No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT387080.4336422498490968No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA368680.41302889499422607No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT351530.3938159039202567No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG246670.2763421870679878No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC192690.21586887755353534No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG186090.20847495679037517No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT184390.20657046204834908No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159900.17913453485292594No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG153640.172121513038171No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA138530.1551939156546331No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC134300.15045508462006227No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT117470.13160058667400384No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT104590.11717123827559427No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC94010.1053185592340436No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT91210.10218174436482412No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT89660.10044529327650618No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG24350.016.03085
TAATACT21100.014.2037924
ACGGACC26450.014.1984878
GACGGAC26350.014.1821637
TCTAGCG18500.014.128
CAAGACG29950.013.9599324
CGAGCCG27850.013.81687515
CGCAAGA27750.013.7333332
AGTACCG6600.013.4545455
CTAGCGG19700.013.42893429
CGGACCA28650.013.3664929
CGAACGA13450.013.06691516
TAACGCC18700.013.0588234
GTTCAAA35300.012.8923511
TTAGACT7950.012.7987414
GCGCAAG30500.012.7983611
AGACGGA29950.012.786316
CGGTCCA21750.012.58850610
TTAACGG5000.012.5835
ACGAACG13850.012.55595615
Produced by FastQC (version 0.11.5)