FastQCFastQC Report
Thu 28 Apr 2016
ERR1042463.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042463.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13580709
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT733590.5401706199580596No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT652870.48073336966427893No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT553690.4077033091571287No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC289110.2128828472799174No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260500.1918162004649389No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG233430.17188351506537694No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA192680.14187771787172526No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT160590.11824861279333795No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG147320.10847739981763838No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG26000.017.36153828
GACGGAC38550.016.796377
ACGGACC40900.016.0574598
CGCAAGA41550.015.9843562
AAGACGG43150.015.9490165
CTAGCGG28500.015.70877229
CGGACCA42450.015.4275629
TCGTTTA24100.015.12240730
AGACGGA43200.015.0740756
CAAGACG46650.015.0300114
GTAAACG28700.014.56794527
TAAACGC29000.014.28965528
TATACTG19000.014.1184215
CGCAATA31350.014.10366836
CGAGCCG40950.013.77899915
CGGTCCA34750.013.62877710
GCGAAAG46550.013.55209518
TAACGCC28300.013.5318034
CGAACGA15750.013.507936516
GAGCGAA48200.013.47199216
Produced by FastQC (version 0.11.5)