FastQCFastQC Report
Thu 28 Apr 2016
ERR1042462.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042462.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4842157
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC257300.5313747571588447No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG232370.4798894377030733No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA170540.35219841074958946No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT154210.31847377109003283No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG144020.2974294307268434No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT127920.26417978599206926No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT105900.21870418493245883No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC95110.19642072737418467No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT94020.19416966446978073No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG94020.19416966446978073No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG81600.16851993853152636No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC74200.15323749312548107No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT66480.13729418521539058No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA64550.13330835823786796No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT50510.10431301587288475No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGG1052.2639142E-819.3809524
TTAACGG5200.017.43269235
GACGGAC8900.016.8370787
ACGGACC9450.016.2486788
CGGACCA9250.016.29
TTTAGCG4350.016.16092126
TAACGGC5650.016.04424936
GTACTAG2150.015.4883721
AAGACGG10200.015.4166675
GTATTAG19900.015.2462311
AATACTG10150.015.1280795
CGCAAGA9650.014.7616592
AGACGGA10400.014.5865386
CTCGCTA3050.014.55737613
TCTAGCG7200.014.38888828
TCGCTAA2850.014.28070314
TGTACTA4550.014.230775
GCATAAG6550.014.1221381
CTAGCGG7350.014.09523829
AATCGTC3350.013.80596928
Produced by FastQC (version 0.11.5)