FastQCFastQC Report
Thu 28 Apr 2016
ERR1042461.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042461.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10112027
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1171911.158926889732395No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT950500.9399698003179777No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT845800.8364297286785329No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC617190.6103524051112601No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG475280.47001456780129247No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA407620.4031041451926503No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT401950.3974969607972763No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG296710.2934228715963674No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG222880.22041080388729184No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT219470.21703858187878652No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC211950.2096018928746927No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG185930.1838701577833999No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA153670.15196755309296542No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC153300.15160165217122146No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA146850.14522310907595481No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT141120.13955658939597373No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132230.13076507806001705No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT118210.11690039989015061No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC107920.10672439858002752No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT106610.10542891153277183No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102860.10172045624482608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG20050.016.1471315
TCTAGCG13100.015.67557128
CAAGACG22300.015.0986554
GACGGAC19950.015.0225567
ACGGACC21150.014.8699778
CTAGCGG14500.014.54482729
CGGACCA22300.014.4349789
GCGCAAG22700.013.9361231
CGCAAGA22650.013.8035322
TATACTG10650.013.5492955
TTAACGG3450.013.40579735
AGACGGA22550.013.2904666
CGCAATA16150.013.17337536
TGCGTAT1551.8914707E-613.12903210
CGGTCCA17200.013.01453610
ACAACAC48300.012.6780543
GTCCTAT16700.012.6287421
CGAGCCG20950.012.62768615
GTAAACG16200.012.561728527
TAAACGC16750.012.48059728
Produced by FastQC (version 0.11.5)