FastQCFastQC Report
Thu 28 Apr 2016
ERR1042460.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042460.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17435990
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1080590.6197468569321272No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT852940.4891835794812913No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT789490.4527933314942254No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC498380.2858340707926536No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG366310.2100884435010573No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT352050.2019099575074315No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA326330.18715885934782023No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG221260.12689844396561364No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT181330.1039975361307273No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC179640.10302827657047292No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG174860.1002868205361439No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGAC43050.017.1893167
AAGACGG48000.016.8041675
ACGGACC45950.016.5875958
CGGACCA48000.015.8791679
TCTAGCG29150.015.86620828
CAAGACG51450.015.5335284
CGCAAGA51000.015.0176472
AGACGGA51150.014.9012716
CTAGCGG31500.014.829
TAAACGC38900.014.21979428
CGCAATA33950.013.89543536
CGAACGA20900.013.72009616
GTAAACG40000.013.69000127
GCGCAAG57950.013.6315791
TTCGGGC41500.013.32891635
TCGTTTA32150.013.11975230
CGAAAGC56650.013.06266619
GCGAAAG56950.013.02633918
CGAGCCG51350.013.00584215
GTATTAG47100.012.9225051
Produced by FastQC (version 0.11.5)