FastQCFastQC Report
Thu 28 Apr 2016
ERR1042459.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042459.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15282047
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT983480.6435525293175711No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT835350.5466217974594634No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT706570.4623529819009194No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC526210.3443321434621946No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG409950.2682559476488981No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350400.2292886548510157No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA344490.22542137188820321No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG260450.17042873902952924No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG187470.12267335652089016No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT186590.12209751743336478No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC184850.12095892651030322No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT171400.11215774954755735No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG159530.1043904654919593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG44100.017.4092985
TCTAGCG28250.017.02654828
GACGGAC43800.016.8105017
ACGGACC44500.016.7123588
TTAACGG8150.016.34355735
CTAGCGG30000.016.15666829
CGCAAGA46250.016.122
CAAGACG51050.015.7277174
CGGACCA47800.015.5585789
AGACGGA49100.015.33503156
GCGCAAG52200.014.7787361
TAAACGC44150.014.33069128
TATACTG19450.014.2673525
CGGTCCA35350.014.23479610
CGAACGA18500.014.216
GTAAACG44000.014.16931827
CGCAATA34150.014.0849236
TAACGGC9350.014.04812836
ACGAACG19150.013.81462215
GCGAAAG53900.013.48886918
Produced by FastQC (version 0.11.5)