FastQCFastQC Report
Thu 28 Apr 2016
ERR1042458.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042458.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10226567
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT528760.5170454562122362No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC460650.4504444160000125No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT429220.41971073968419703No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT373220.36495140549120736No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG369090.36091290459447434No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA297800.29120231647629163No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG232340.22719256618570044No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175330.17144560828673006No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG164270.16063063978361458No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT159580.1560445455449517No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC152180.14880849066944948No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG136770.13373989531384284No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT129140.126278936030048No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC118040.11542485371679469No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT112570.11007604018044374No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA108030.10563662272979779No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT106580.10421874711230074No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGG4150.022.28915626
TTTAATT56950.021.34240525
CCGAACG4200.019.3809525
ATGCGTT5550.019.33333429
GCGAACG3800.018.01315925
TTTTAAT70600.017.66147224
AAGACGG34150.017.5519775
ACGGACC35450.016.5430188
AACGGCG6750.016.44444528
GACGGAC35550.016.3924057
CAAGACG38500.016.3376624
GCTAACC12300.015.943089525
CGGACCA36800.015.936149
CGCAAGA37300.015.7721192
AATGCGT6650.015.57894828
TGCGTAA3500.015.32857123
TTAATTT81500.015.29938626
AGACGGA38250.015.283666
AAAACGC9700.015.0670128
GCGCAAG40200.014.9104471
Produced by FastQC (version 0.11.5)