FastQCFastQC Report
Thu 28 Apr 2016
ERR1042457.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042457.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8470090
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC480080.5667944496457535No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG413430.4881057934449339No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT318420.3759346122650409No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA316460.3736205872664871No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT277680.3278359497951025No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG263390.310964818555647No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT233500.2756759373277025No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG169460.20006871237495705No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT168510.19894711862565806No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC157430.18586579363383388No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG146040.17241847489223847No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC130720.15433129990354294No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG121850.14385915616008804No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT119210.14074230616203606No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA114650.13535865616540083No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT106320.12552404992154748No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105980.12512263742179836No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC105900.1250281874218574No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT94500.11156906243026934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGG3000.020.96666726
CCGAACG2600.019.21153625
TTTAATT41800.018.89832525
GCGCGAA2850.018.8245623
TGCGTAA3000.018.523
AATGCGT4300.017.63953428
TGAATCC19650.017.1348626
CGCGCGA1950.017.07692322
AAGACGG28900.017.0276815
GCTAACC10350.016.62318825
ACTGAAT20550.016.2043824
TTTTAAT49100.016.01323724
GAATCCC18050.015.98891927
CAAGACG32100.015.9641754
CGCAAGA30600.015.9003272
GACGGAC29000.015.8206897
ACGGACC29250.015.5589748
AAAACGC9050.015.33149128
AACGCGA2550.015.23529523
ACGCGAA2800.015.19642924
Produced by FastQC (version 0.11.5)