FastQCFastQC Report
Thu 28 Apr 2016
ERR1042456.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042456.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6520459
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC424280.6506903885140601No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG350440.5374468269795117No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT327190.501789827986036No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT277740.4259516086214176No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA277070.42492407359665935No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT239140.3667533221204213No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG217940.33424027357583264No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG151870.23291305105974902No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT151570.23245296075015578No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC137710.2111967884469483No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG132210.20276179943773898No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113910.1746962905525516No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC113470.17402149143181483No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA105110.1612003081378167No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT103470.158685147778707No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG80190.12298213975427191No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC74520.11428643290295973No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT74290.11393369699893825No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG70890.10871934015688159No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC68690.10534534455319787No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC67780.10394973728076505No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAATT36500.020.17260225
CAACGCG4400.019.34090822
ACGCGTA500.007038322718.524
ATGCGTT4300.018.06976929
GAATCCC12100.017.88843227
TGAATCC16600.017.49698826
CTAGTAC3300.017.3787883
TATAACG5550.017.02
ACGCGAA3500.016.91428624
CGCAAAG13950.016.84229525
TTTTAAT44750.016.78435924
ACTGAAT17150.016.39650224
TAACGCA6350.016.314964
TTAATTT46400.016.22737126
AACGCGA3800.016.06578823
GACGGAC16450.015.7446797
AATGCGT4150.015.60240928
CGAACGG1802.0190782E-1015.41666626
AAGACGG18600.015.4166665
ACGGACC17050.015.2991218
Produced by FastQC (version 0.11.5)