FastQCFastQC Report
Thu 28 Apr 2016
ERR1042455.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042455.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9478668
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC388820.41020531576799607No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG313630.3308798240427875No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA245730.25924528636302063No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT231860.24461242866613747No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT194920.20564070816701246No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG194010.20468065766202592No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT172850.18235684591970094No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG130060.13721337217423377No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC129520.1366436718745714No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT127590.13460752080355595No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG110680.1167674614196847No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT104760.11052185813449737No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT104180.1099099578126378No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC102130.10774720667503072No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG99810.10529960538759243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGG4250.019.1529426
ATGCGTT5850.016.76068529
AAGACGG31050.016.206125
AATGCGT5750.016.08695628
TTTAATT40400.015.88985225
GAATCCC16150.015.80804927
ACGGACC31000.015.3967738
GACGGAC30850.015.3517037
TATACTG14650.015.0273055
TAATACT20250.014.84
CGCAAGA33500.014.7447762
CAAGACG35150.014.5789474
GCGCAAG35450.014.4555711
TGCGTAA3400.014.14705923
TCTATAC11250.014.1422223
CGGACCA34800.014.1408039
CAATGCG7500.014.05999927
TCTAGCG22550.014.02882428
AGACGGA35000.013.9014296
CGGTCCA27800.013.7751810
Produced by FastQC (version 0.11.5)