FastQCFastQC Report
Thu 28 Apr 2016
ERR1042454.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042454.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5953362
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT596981.0027611289217757No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT555260.932683078905667No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC456770.7672471453944846No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT424220.71257215670742No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG375230.6302825193562898No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA295250.4959382614395026No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG235460.39550761401708817No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179660.30177906198212034No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC168460.28296616264893687No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT156530.26292706541278693No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG156520.2629102681812394No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT141880.23831912119572102No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG141380.23747925961834673No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC115830.19456233301452186No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA102280.17180208426767934No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT102190.17165090918375198No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT101830.1710462088480425No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG95640.1606487225201491No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA95300.16007761664753462No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA92650.15562635028745103No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC90950.15277082092437852No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG90770.15246847075652378No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCC90370.15179658149462438No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT86500.1452960528857476No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC86370.14507768887563027No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC85710.14396907159349626No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC82240.13814043224651887No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA81690.13721658451140717No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG77940.1309176226811002No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGG76760.12893554935849694No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG76650.1287507798114746No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC74160.12456826915615077No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC73330.12317409893770949No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG72930.12250220967581008No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG72670.12206548165557544No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGG70790.11890760212464822No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA69990.11756382360084941No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG67920.11408679667051995No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC67910.11406999943897246No Hit
GAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATG63340.1063936646217717No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAA62520.10501629163487791No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG61700.10363891864798411No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC61330.10301742108072716No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC60710.10197599272478307No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT59790.10043064742241442No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC59760.10038025572777197No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGG59700.10027947233848707No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGTT2800.029.73214129
AAGTATC3250.029.628
CGTGTAA702.0736479E-1029.07142823
CGCGCGA900.028.77777722
TTTAATT40900.028.63202925
CGAACCG851.8189894E-1228.29411726
ACTGAAT10350.028.06280324
GAATCCC8950.027.4916227
GCGCGAA1150.027.34782623
TGAATCC11050.027.28959326
CGAACGG2400.026.97916626
GCCGAAC1800.026.72222124
TTTTAAT46800.025.25961724
GCGAACC1150.024.13043625
TGCGTAA1150.024.13043623
CCGAACG2400.023.89583225
GCGAACG1400.023.78571325
CGTAACG551.9043919E-523.54545425
AACGGCG2600.023.4807728
AACGCGC952.8194336E-1023.3684228
Produced by FastQC (version 0.11.5)