FastQCFastQC Report
Thu 28 Apr 2016
ERR1042453.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042453.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13054767
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC660420.505884172425291No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG573270.4391269487996224No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA428720.3284011120229109No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT359870.27566175635306245No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG352680.2701541896534806No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT306330.23464991753587022No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT265980.20374166769885665No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT225240.17253467641360432No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG223130.17091840857826113No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC219970.16849783684381345No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT215670.1652040208760524No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG202290.1549548911903215No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT184460.14129704497981466No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC181190.1387922128368894No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC158600.1214881889504424No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA157910.12095964638817376No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT155340.11899101684465145No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCC142380.10906360871856234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGG5600.021.80357226
AAGACGG53250.020.0112695
GCGCGAA3900.018.523
GACGGAC56750.018.157717
GAATCCC20250.017.99753227
ACGGACC57850.017.9723438
CAAGACG61500.017.8682924
CGCAAGA58450.017.8511542
CAACGCG6650.017.52631622
CGGACCA58950.017.4486859
CCGAACG5550.017.33333425
AGACGGA61000.017.1049186
ATGCGTT6950.017.0359729
TCTAGCG40900.016.9168728
TTTAATT50450.016.79484725
GCGAACG5150.016.52427325
CTAGCGG42050.016.41022729
CGGTCCA47850.016.31556910
GCGCAAG65950.016.0735421
TGAATCC26600.015.92669126
Produced by FastQC (version 0.11.5)