FastQCFastQC Report
Thu 28 Apr 2016
ERR1042452.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042452.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9162664
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC347360.3791037191803607No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT345660.37724836357635727No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT334720.36530860457177083No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG294460.32136941832637317No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT268810.2933753764189105No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA236850.258494691063647No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG188600.20583533347943347No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151130.1649411131959002No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT134950.14728249338838573No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG129630.14147632173350458No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC114580.12505096770982763No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG111450.12163493062716257No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT98920.10795986844000828No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC95380.1040963632410836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGAC26000.017.1480777
AAGACGG28250.017.0920375
ACGGACC27000.016.7185178
CAAGACG31150.015.9165334
CGCAAGA28800.015.6736112
TCTAGCG16700.015.28742528
CGGACCA29350.015.1908019
CTAGCGG16950.014.95280229
AGACGGA30450.014.8850576
TATACTG10550.014.3791485
CGAACGA9450.013.89947116
GCGCAAG33200.013.8751
TATAACG6150.013.5365852
TAAACGC18350.013.4087228
GCAAGAC40650.013.3800743
CGCAATA19500.013.1871836
GTAAACG18450.013.13550227
GCGAAAG33450.013.10762318
GAGCGAA33950.013.02356416
AGCGAAA34300.012.89067117
Produced by FastQC (version 0.11.5)