FastQCFastQC Report
Thu 28 Apr 2016
ERR1042451.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042451.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10177946
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC292420.2873074783458273No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG237800.23364242647779818No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA193870.19048047611964142No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT176020.17294255638613135No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT169340.16637934608810068No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG149860.14723992444055017No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT141780.13930119102616578No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT115910.11388348886897219No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGAC27400.016.3394177
AAGACGG31050.016.0273745
ACGGACC28250.015.9787618
TCTAGCG19950.015.67167928
CAAGACG32150.015.1912914
CGCAAGA30350.015.1169692
CGGACCA30250.014.7388429
TCTATAC11350.014.6696033
CTAGCGG21650.014.61200929
TATACTG13700.014.5839425
TTAACGG5550.014.33333335
AGACGGA32600.014.1871176
GTATTAG28650.013.9476451
GCGCAAG33550.013.6751121
TAACGCC18150.013.4545464
ATAACGC21450.013.4545463
TAACGGC5700.013.30701736
CGAACGA11050.013.05882416
CGCAATA24150.013.02277436
TAAACGC20050.013.00997528
Produced by FastQC (version 0.11.5)