FastQCFastQC Report
Thu 28 Apr 2016
ERR1042450.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042450.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12361897
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT228670.18497970012207673No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC200120.1618845392418332No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT196100.1586326111599215No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT181480.14680594733963565No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT166070.1343402230256408No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG155120.12548235922043358No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT142580.1153382850544702No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA128750.10415068172789338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG40550.017.7472255
GACGGAC39600.017.0517677
TCTAGCG27400.016.67700828
CAAGACG44050.016.5051084
CTAGCGG27800.016.4370529
ACGGACC42750.016.0549728
CGCAAGA43950.015.9533562
CGGACCA44100.015.5215439
AGACGGA45200.015.3484526
TAACGGC7500.015.04666836
GTATTAG41550.014.4259931
CGCAATA33250.014.35488736
GCGCAAG49050.014.2945981
TTAACGG7650.014.26797435
CGGTCCA33800.014.12130310
TATACTG16500.013.903035
TAGCGGC33650.013.85438430
GCAAGAC62400.013.6378213
TATAACG4500.013.5666672
CGAACGA15650.013.47603716
Produced by FastQC (version 0.11.5)