FastQCFastQC Report
Thu 28 Apr 2016
ERR1042449.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042449.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11290375
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT218570.19358967261937712No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT172280.15259014868859538No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT159800.14153648572345914No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC149140.13209481527407194No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT148440.13147481815262999No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT136720.12109429491934502No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG113210.10027124874063084No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG37450.016.1535385
TCTAGCG26450.015.52741128
GACGGAC38500.014.87
CGCAAGA39900.014.7443622
CTAGCGG27950.014.69409729
TATACTG14400.014.6458335
ACGGACC39600.014.529048
CAAGACG42100.014.3254174
CGGACCA41450.014.1483729
GTATTAG45250.014.0640891
CGGTCCA31600.013.87510
TAACGGC6150.013.837399536
GTCTATA6100.013.3442621
TCTATAC11700.013.2820513
CGCAATA31350.013.27751336
GCGCAAG46050.013.2171551
AGACGGA44150.013.1574186
CGAACGA14950.013.11705616
GTTCAAA49250.013.0720811
TCTAGAC10100.013.0049513
Produced by FastQC (version 0.11.5)