FastQCFastQC Report
Thu 28 Apr 2016
ERR1042448.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042448.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4816990
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT181200.37616852017546226No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT159650.331431038885279No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT128720.26722081631890454No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC121670.25258512058360094No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG96520.2003740925349648No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA81190.16854923925521953No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66180.13738870124289235No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG61030.12669737740788334No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT49080.10188935414024111No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC1156.4173946E-817.6956523
ACGGACC12800.016.6210948
TCTAGCG7300.016.47260328
GACGGAC12850.016.2684827
CGGACCA13450.015.955399
AAGACGG14200.015.5035225
CTAGCGG7800.015.41666729
AGACGGA14750.014.4237296
CAAGACG16050.014.0623054
CGCAAGA15350.013.8599352
ACGAACG5750.013.83478315
TAAACGC10300.013.83009728
TATAACG2850.013.6315782
CGAACGA6150.013.53658516
GTATTAG12450.013.5220871
GCGAAAG15450.013.41100418
GAGCGAA15750.013.39047616
CGAGCCG14950.013.36454815
AGCGAAA15850.013.30599317
TAACGAA6700.013.25373213
Produced by FastQC (version 0.11.5)