FastQCFastQC Report
Thu 28 Apr 2016
ERR1042447.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042447.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6376877
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT484870.7603565193432459No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT459260.7201957949008582No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT362740.5688364382753501No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC353060.5536565939722532No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG322400.5055766325742209No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA242330.38001360226957487No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG211810.33215318407427336No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181760.28502980377385356No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT140180.21982547256282348No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG138110.21657936949387607No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG121400.19037532008222832No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC114740.17993133629518024No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC99250.15564044907875751No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT98830.1549818194705653No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA93460.1465607694801076No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA82540.1294363996671098No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG70320.11027341440018366No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC67240.10544346394010737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGAC10500.017.7952377
ACGGACC11150.016.9237678
TCTAGCG5600.016.84821328
AAGACGG12300.016.5447165
CTAGCGG5800.015.94827629
CTAACGC700.002594228115.8571423
CGCAAGA12400.015.3669362
CGTATTA5550.015.33333315
CGGTCCA7500.015.29333410
CGGACCA12550.015.1832689
TTAACGG5500.015.13636335
GTATTAG22050.014.934241
TACTCGC5600.014.86607120
TCCGTAT5700.014.60526313
TATACTG7100.014.3309865
TAGCGGG6500.014.23076928
TAATACT19200.014.1640634
TAACCGG4450.014.1348322
CGTTCGG1458.927509E-714.03448324
CCGTATT5800.014.03448314
Produced by FastQC (version 0.11.5)