FastQCFastQC Report
Thu 28 Apr 2016
ERR1042446.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042446.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11079312
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT849330.7665909218911788No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT774210.6987888778653404No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT623510.5627696015781485No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281830.25437500090258314No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT169380.15287952898158297No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA126440.1141226097793798No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG23800.019.1218528
AAGACGG34450.018.9564595
CTAGCGG25700.017.92412429
CGCAAGA36150.017.7067782
ACGGACC36450.017.4087818
GACGGAC36950.017.123147
CAAGACG42100.016.8301664
GCGCAAG40800.016.4142151
CGGTCCA28850.016.0953210
TCGTTTA23450.016.09381730
AGACGGA39900.016.0889726
CGGACCA40050.015.8439469
CGCAATA28800.015.73784836
TAGCGGC30100.015.48837130
TAACGCC27400.014.9890524
GGTATCA401350.014.8377971
TATACTG12850.014.8287945
CGCATCG26650.014.57786213
CGAACGT10850.014.4930884
CCGGTCC33150.014.4539989
Produced by FastQC (version 0.11.5)