FastQCFastQC Report
Thu 28 Apr 2016
ERR1042445.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042445.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15991008
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT903190.5648111738797204No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT728780.45574362791889045No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT665520.4161838953491862No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293450.18350938227283733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA307550.020.6624951
ACGGACC35300.018.6048168
AAGACGG36400.018.4491755
GACGGAC36400.017.8392857
TCTAGCG21600.017.0439828
CGGACCA40250.016.638519
CGCAAGA40100.016.2855382
CTAGCGG23250.015.9139829
AGACGGA41500.015.6915666
TCGTTTA28250.015.52035430
TAACGCC31450.015.2941184
CGAACGA18300.015.26502616
GCGAAAG44600.014.31053818
AATAACG32100.014.2928352
ATAACGC35150.014.2105263
GTATCAA448850.014.1578482
GCGCAAG47900.014.1356981
CAAGACG52000.014.1240394
CGGTCCA28600.014.03671310
CGAAAGC45300.014.00772719
Produced by FastQC (version 0.11.5)