FastQCFastQC Report
Thu 28 Apr 2016
ERR1042444.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042444.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19278164
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC867300.44988724029943933No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT813070.42175696814281693No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG773480.4012207801531308No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT743130.38547757971142893No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT645740.3349592834670356No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA576710.2991519316881006No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG489320.2538208514047292No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT324840.16850152327783913No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT316890.1643776865888266No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG287110.14893015745690305No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG270480.14030381731372346No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC269650.13987327838895863No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC241190.12511046176388999No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT212320.1101349692844194No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA209520.10868254881533325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG15000.015.53999935
TAATACT50700.014.8875734
ACGGACC40500.014.8456798
GACGGAC40350.014.7174737
TAACGGC16700.014.06886236
AAGACGG45250.013.8596695
GTATTAG51900.013.7591521
TCTAGCG26550.013.72693128
CGGACCA44900.013.5144769
GGGGTTA34050.013.3656396
CTAGCGG27450.013.27686829
ATAACGC43850.013.2052463
CGCAAGA46800.013.20299152
TAACGCC35100.013.0712254
AGACGGA46850.013.0704386
CAAGACG51600.013.0145354
TATACTG28650.012.9790575
GTAAACG29950.012.97161927
TATACCG23250.012.9698936
TAAACGC31300.012.53035228
Produced by FastQC (version 0.11.5)