FastQCFastQC Report
Thu 28 Apr 2016
ERR1042443.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042443.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18563929
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT712100.38359336539156125No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT578940.31186286049682693No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT526380.2835498886038618No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT247240.1331830131433922No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222220.11970526282448074No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT199850.10765501203974653No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG56150.018.3187895
CAAGACG57900.017.7651124
GACGGAC55950.017.491517
ACGGACC57650.017.2324378
TCGTTTA43650.016.99541930
TCTAGCG38600.016.91839228
CTAGCGG40700.016.0909129
CGCAAGA62600.016.0766772
AGACGGA62050.016.0402896
CGGACCA63050.015.8445679
GCGCAAG68100.015.2400881
GGTATCA352000.015.0627831
CGTTTAT49650.014.90432931
ATAACGC57300.014.7870863
CCGGTCG51850.014.59305720
TAACGCC53650.014.5862074
CGCATCG52850.014.45695413
CGCAATA46750.014.4438536
ATGGTCG47850.014.42110836
CGGTCCA48550.014.213181510
Produced by FastQC (version 0.11.5)