FastQCFastQC Report
Thu 28 Apr 2016
ERR1042441.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042441.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2810606
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT309981.1028938243211606No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT295741.0522285941181369No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT223580.7954868096061847No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC194260.691167669890408No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG155390.5528700927842608No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA126970.4517531094717651No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111880.3980636204434204No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG97070.34537035785165193No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC73910.2629681997405542No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG69430.2470285767553332No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT65450.23286792954971278No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG58690.20881617700951324No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC48280.17177790127822967No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA46840.16665445103298007No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA44290.15758167455701724No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT43810.15587385780860072No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT38200.13591374956148247No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG34650.12328302152631852No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT33330.11858652546817305No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC33160.11798167370310887No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG30990.11026091881964246No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30490.1084819430400419No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC29470.10485283244965676No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC28710.10214878926466393No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCT500.007037350418.54
TAACGAA2900.017.86206813
AATGCGA1401.873559E-917.17857220
ACGAACG3350.017.11940415
CGAACGA3350.016.01492716
ACAACAC11350.015.9735683
CGCGATA700.002593743615.85714314
CGAGCCG6500.015.65384615
ACGTTTA957.06548E-515.57894826
ACGGACC6650.015.5789488
CTAGCGG3850.015.37662329
CAGTTCG1701.4897523E-915.2352949
TAGTACT2550.015.2352944
ATAACGA3450.015.01449212
CCGATAA3750.014.8000019
TTACTCG1001.094257E-414.819
ATACGGC750.004106368414.86
CTAGACA2750.014.7999994
TCTAGCG3900.014.70512728
TCCGATA3650.014.698638
Produced by FastQC (version 0.11.5)