FastQCFastQC Report
Thu 28 Apr 2016
ERR1042440.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042440.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3380545
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC247140.7310655530395247No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG197590.5844915538766678No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT189050.5592293550300321No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT172360.5098586174714431No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA158890.4700129712812579No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT136860.4048459641862481No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG124340.36781051576003276No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC85800.25380522963013363No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG79710.2357903829116311No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT78110.23105741825652373No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG71160.210498603035901No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71110.2103506978904289No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC60970.18035553438868585No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA55440.16399722529947094No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT53470.15816976256786996No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG44600.1319313897611184No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT39110.11569140478828119No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC38050.11255581570427255No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC36930.10924274044569737No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG35820.10595924621621662No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC34150.10101921435744829No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC656.907197E-519.9230773
CGTATAC1355.638867E-1119.1851863
TATAACG2150.017.2093032
CAAGACG8350.017.059884
TATACTG6100.016.6803285
GACGGAC7550.016.662257
ACGGACC7800.016.3653858
AAGACGG8300.016.2710845
TCTAACG700.002593890515.8571422
GCGTTAT5000.015.541
TCTATAC2950.015.0508473
CGCAAGA8650.014.9710982
AGACGGA9000.014.1833336
CGGACCA9050.014.1049729
CTACGCT1203.304086E-513.8750014
CGAGCCG7600.013.87515
CGAACGA4550.013.82417716
GTAAACG6800.013.602941527
TTCGGGC6950.013.5755435
GTATAAC4300.013.3372111
Produced by FastQC (version 0.11.5)