FastQCFastQC Report
Thu 28 Apr 2016
ERR1042439.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042439.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6296273
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT293860.4667205503954482No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT252620.4012214845194927No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT214730.3410430265650806No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC145800.23156556267493483No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG111510.17710477293471868No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109050.17319769965501813No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA91980.1460864228727058No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG72180.11463924769462824No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT65600.10418862079201459No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG18200.017.0769235
TTAACGG2850.016.87719335
GACGGAC17450.016.7507177
ACGGACC17700.016.5141248
TCTAGCG11500.016.08695628
CAAGACG19950.015.9498754
CTAGCGG11800.015.83474529
CGCAAGA19100.015.594242
CGGACCA19400.015.4484549
TAACGGC3200.015.0312536
AGACGGA20400.014.9632356
GTATTAG16250.014.81
TATACTG10450.014.51674655
TAACGCA4350.014.4597694
CGAACGA8000.014.33750116
AATCGGG2750.014.12727423
CGGTCCA13550.014.06273110
ACGAACG8550.013.63157915
GTATAGG10100.013.5544561
TACGACG12200.013.4959025
Produced by FastQC (version 0.11.5)