FastQCFastQC Report
Thu 28 Apr 2016
ERR1042438.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042438.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7473354
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT330700.4425054667556227No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT296560.39682316667991374No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC281640.3768589043152512No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG218410.2922516449776098No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT210140.2811856630904946No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA196800.2633355786438057No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG148380.1985453920689425No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121770.1629388892858548No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG101380.13565528944567593No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT100600.13461158135958767No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT89690.12001304902725068No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC89210.11937076712811945No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG87420.11697559087927589No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG23750.018.227375
CAAGACG24750.017.78994
ACGGACC24350.017.3223828
GACGGAC24150.017.312637
CGCAAGA25850.017.1760162
AGACGGA27100.015.8376396
GCGCAAG28150.015.7069271
CGGACCA27450.015.5009119
TATACTG12200.014.8606555
TCTAGCG13450.014.44237928
TCGTTTA16700.014.40119730
GCAAGAC34550.014.3502173
ATAACGC21450.014.3170163
CTAGCGG13900.013.9748229
TAACGCC17950.013.8105854
CGCATCG17650.013.62606313
AACGCCG18750.013.4186675
TAAACGC16600.013.15060228
GCGAAAG31800.013.14779918
TATAACG5500.013.1181812
Produced by FastQC (version 0.11.5)