FastQCFastQC Report
Thu 28 Apr 2016
ERR1042437.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042437.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13455665
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC772150.5738475207282583No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT661160.49136181675153184No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG615810.4576585401018828No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT592640.44043902698231563No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA502990.3738128141567139No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT484430.36001936730737577No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG386750.2874254078115054No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT255820.1901206666485826No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG251400.18683580484502252No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251030.18656082772571997No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC246850.18345432945900483No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG222520.16537272591135407No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC194560.14459337386892437No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA176630.13126813130380402No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT165980.12335324935631202No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG154180.11458370879477157No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC136630.10154087516298899No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG35150.016.684215
GACGGAC33800.016.1464487
ACGGACC34400.016.0261638
CAAGACG39200.015.7627544
CGCAAGA37700.015.4084882
CGAGCCG36150.015.1991715
CGGACCA37500.014.8986669
TATACTG20600.014.3689325
TATAACG9400.014.3670212
GCGCAAG40500.014.3432091
GTACTAG7750.014.3225811
AGACGGA39800.014.2701016
TCTAGCG20450.013.84107628
TACCGCA39400.013.61675128
CTAGCGG20850.013.5755429
ATAACGC31450.013.4705893
ATACCGC41100.013.00851527
GCGAAAG41750.012.93892218
TACCGTC22250.012.887647
GTAAACG29600.012.812527
Produced by FastQC (version 0.11.5)