FastQCFastQC Report
Thu 28 Apr 2016
ERR1042436.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042436.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10062307
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT217060.21571593870073733No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT163120.16210994158695416No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT141700.14082257677091348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67700.026.807241
GTATCAA104000.017.468272
TATACTG17750.014.27887255
ATCAACG137500.013.225824
TATCAAC142400.013.1214893
TCAACGC138900.013.1058325
TAATACT18700.012.8609624
CGTATAC2900.012.7586213
CAACGCA143200.012.7510486
GTATACG3850.012.4935061
CTAGTAC5700.012.3333333
GTATTAG15150.012.3333321
TTATACT19100.012.1073294
TAGACCG2307.932613E-912.0652185
AACGCAG152550.012.0180277
TCTATAC9550.011.8167533
ATACCGT8000.011.7937516
TTAGACT10250.011.5512194
GTGTAAG16050.011.0654211
TTAATAC13600.011.0183823
Produced by FastQC (version 0.11.5)