FastQCFastQC Report
Thu 28 Apr 2016
ERR1042435.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042435.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6388631
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT69710.1091157088271337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40500.026.5851841
GTATCAA60500.017.7355372
TATACTG7750.015.754845
TCTAACG2350.014.9574472
GTATTAG12150.014.6172851
GTACTAG3200.014.4531251
TAACGCC4150.014.265064
ATCGTAC1702.4427209E-814.14705925
CTAACGC2304.0017767E-1113.6739133
CTAGTAC4750.013.6315783
ATCGATA8900.013.3033723
TAATACT12800.013.2968754
GTAATCG9000.013.15555620
TAATCGA9000.012.9521
ATCAACG82600.012.9007264
TCAACGC84550.012.5813125
TCTAGAC6200.012.5322583
TATCAAC87100.012.5315743
CTAATAC11400.012.4956143
AATCGAT9250.012.422
Produced by FastQC (version 0.11.5)