FastQCFastQC Report
Thu 28 Apr 2016
ERR1042434.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042434.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207771
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC3590.17278638501042012No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC3180.15305312098416046No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA3010.14487103590010156No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGA303.589424E-430.83333412
GAGCTAG250.00548611229.61
GTCTAAA250.00548611229.61
GTTCAGA250.00548611229.66
GTTAATT358.8448584E-426.428572
TGAGCGG358.8448584E-426.4285710
ACGAACG358.8448584E-426.4285715
CGAACGA451.3181212E-424.66666616
TAATCAT400.001925895123.1255
TCCGATA400.001925895123.1258
CCGATAA400.001925895123.1259
GGTTAAT400.001925895123.1251
CGATAAC400.001925895123.12510
CGAGACT502.6917903E-422.220
GATAACG450.003815424220.55555511
TTCCGAT450.003815424220.5555557
TAACGAA450.003815424220.55555513
CTCGGAG450.003815424220.55555512
AACGAGA450.003815424220.55555518
ACGAGAC450.003815424220.55555519
Produced by FastQC (version 0.11.5)