FastQCFastQC Report
Thu 28 Apr 2016
ERR1042433.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042433.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7968936
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT486610.6106335902308665No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC453120.5686079044931469No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT423360.5312628938166902No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT382170.47957468851550566No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG311560.3909681292458617No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA290740.36484168024438896No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210850.2645899025917638No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG190730.239341864459697No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG161230.2023231206775911No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC154110.19338842726306246No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT151460.19006301468602585No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG128380.16110055344904262No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA118510.14871496019041938No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC113550.1424907917443433No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA100790.12647861646774425No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT94310.11834704156238673No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG81000.1016446863169688No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79880.10023922892591934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG8750.020.7200012
TAACGCA10850.017.7327184
GTATAAC13400.017.6716421
CGAACGA9500.014.99473716
AAGACGG20400.014.9632355
CAAGACG21400.014.9556074
TAATACT11500.014.6391314
ACGAACG9500.014.60526315
ATAACGC23500.014.5638283
ACGGACC20100.014.3582098
TCTAGCG14900.014.27852328
TATCACG9750.014.2307692
CGCAAGA22500.014.2244432
GACGGAC20050.014.0249397
CTAGCGG15450.013.77022629
CGCAATA16550.013.52568136
GCGCAAG24100.013.4336111
TAACGAA10850.013.12903113
TATACTG12300.013.0853655
AACGAAC10800.013.01851814
Produced by FastQC (version 0.11.5)