FastQCFastQC Report
Thu 28 Apr 2016
ERR1042432.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042432.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10086309
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC451540.4476761519005615No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT450280.4464269337772618No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT393380.3900138296377793No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT364170.3610537809222383No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG296450.29391326400965906No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA286720.284266524057512No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197200.19551255072593948No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG179150.17761700538819503No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC157120.15577551708955178No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG149970.148686699961304No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT141040.13983311437315674No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG124100.12303807071546193No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT118050.11703984083771377No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT115660.1146702921752645No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA113650.11267749183571513No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC107960.10703618142176687No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG105170.10427005557731772No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG8450.016.6390512
AAGACGG30550.016.471365
CAAGACG30950.016.1389354
TATCACG10500.014.97619152
CGCAAGA33400.014.67814352
GACGGAC31850.014.6373627
ACGGACC33200.014.4322298
AGACGGA35050.013.934386
CGAGCCG39050.013.88092115
GTATACG3500.013.7428571
CGGACCA35350.013.7114569
TAACGCA10800.013.5324084
GTATAAC13700.013.503651
TACCGCA39850.013.41656228
CGAACGA14650.013.38566516
ACGAACG14650.013.25938515
GTTCAAA48000.013.2583331
TCTAGAC8300.013.1506023
ATAACGC31700.013.1309153
TACGACG25650.013.1267055
Produced by FastQC (version 0.11.5)