FastQCFastQC Report
Thu 28 Apr 2016
ERR1042430.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042430.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9924543
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT543460.5475919646879458No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT464470.4680013981500206No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT413500.41664386964719685No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC316470.3188761437176503No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG232460.23422740976587034No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219190.22085651702048148No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA201450.20298163855000678No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG136370.1374068307225834No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT123270.12420723049917765No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT114570.11544108378592345No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC114020.1148869020971545No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG109160.10998995117457802No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT99910.10066962277255488No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG30000.017.0199995
CAAGACG30700.016.5716614
TATAACG8050.016.316772
GACGGAC30550.015.8657947
ACGGACC31850.015.682898
CGCAAGA32200.015.5124232
CTAGCGG23100.015.05627729
TCTAGCG23600.014.73728828
GCGCAAG35800.014.4175981
CGGACCA35000.014.3771439
TAACGCA9400.014.1702124
CGAACGA14700.014.09523916
AGACGGA35150.013.8947376
ACGAACG14800.013.87515
CGAGCCG33750.013.86814915
ATAACGC31100.013.8601273
CGGTCCA27750.013.60000110
GTATAAC14300.013.3251761
CGCAATA26450.013.28922536
TCGTTTA21900.013.26255730
Produced by FastQC (version 0.11.5)