FastQCFastQC Report
Thu 28 Apr 2016
ERR1042429.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042429.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10106652
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1269031.2556383657021137No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1087711.0762317728957127No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT943040.9330884253262108No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT497170.4919235370922042No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC379960.3759504136483575No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG275490.27258284939463634No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA241730.23917910698814998No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA191890.1898650512553514No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG168510.16673177230204422No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161910.16020141981736385No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG149200.1476255440476233No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC143320.14180759365218076No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135270.13384254251556302No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT134200.13278383385516787No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT132680.13127987388899903No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT130750.12937024051090312No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG117610.11636890238231218No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111070.1098979167384016No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT108330.10718683100991308No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA105180.10407007186949743No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG29500.018.3745775
CAAGACG30300.018.0726054
GACGGAC29750.017.473957
ACGGACC30550.017.137488
CGCAAGA32350.016.5270482
GCGCAAG34300.016.3965021
CGGACCA32600.016.3435579
TCTAGCG20850.016.05995228
AGACGGA33950.015.5846836
CTAGCGG21500.015.57441829
TCGTTTA22500.014.96444530
GCAAGAC41550.014.5150423
CGCAATA22600.014.48893836
GTAAACG23150.014.30453627
TTCGGGC23950.014.13569835
ACAACAC43500.014.119543
CGAAAGC36700.014.11444219
TAAACGC23600.014.11016928
GCGAAAG36850.014.05698818
GAGCGAA38350.013.8930916
Produced by FastQC (version 0.11.5)