FastQCFastQC Report
Thu 28 Apr 2016
ERR1042428.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042428.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10492023
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1171461.1165244300360377No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT959240.9142564784694048No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT857060.8168682054928779No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC577630.550542064194865No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG491760.4686989344190344No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA385280.3672123097709565No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT385130.36706934401497215No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG308860.29437602262213874No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC197160.18791418966580611No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG194710.18557908231806203No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT188340.17950780321392737No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG170210.1622280088406211No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC155540.14824595790535341No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA132150.12595283102219657No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT127430.1214541752338896No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG121160.11547820663374452No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111830.10658573661151906No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC106430.1014389693960831No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTGT10050.017.85572234
TATAACG7850.016.2611472
CGTTGTA11050.015.90497835
CGTAGCG4200.015.85714215
TAACCGG2950.014.42372922
TATCACG11650.014.2918452
AACCACG13500.014.1148157
GACGGAC10200.013.9656867
ACGGACC10400.013.8758
ACGACCA14200.013.80985911
CACGCAG12750.013.7843135
TCACGCA12350.013.7813764
CCACGAC15100.013.5993399
TAACGGC5450.013.57798236
TTAACGG5700.013.30701735
CGAACGA14800.013.2516
ATCACGC13000.013.2346153
TACTCCG10800.013.18981555
TAATACT32000.013.0656244
GTATTAG20700.013.0483081
Produced by FastQC (version 0.11.5)