FastQCFastQC Report
Thu 28 Apr 2016
ERR1042427.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042427.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10222309
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT289050.28276390392816336No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT256050.25048156928146076No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC235380.2302610887618443No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG196020.19175706780141355No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA184730.18071259634198103No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT163070.15952364578296352No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT153060.14973133760679705No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG150180.14691397021944846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG34100.017.5777135
GACGGAC34600.017.002897
CAAGACG37150.016.8815614
ACGGACC34450.016.8084188
CGGACCA35850.016.255239
CGCAAGA36700.015.9291552
AGACGGA39100.015.1879796
GCGCAAG41800.014.6052631
ATAACGC29450.014.1341263
CGAACGT11600.013.7155184
TTAGAGT19550.013.6265984
GCGAAAG42350.013.54191318
GCAAGAC50050.013.528473
TAACGCC27400.013.3686134
AGCGAAA42800.013.35630917
GAGCGAA43450.013.28423516
CGCATCG26900.013.13568813
CGAAAGC43950.013.04891919
AACGCCG28850.013.0173315
GTACCGT3000.012.956
Produced by FastQC (version 0.11.5)