FastQCFastQC Report
Thu 28 Apr 2016
ERR1042426.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042426.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1181721
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT51490.43572044501197826No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT48990.4145648592180388No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT18940.16027471797488577No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18890.159851606259007No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15250.12904907334303103No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC11850.10027747666327332No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA358.869544E-426.4285714
ACGGACC3500.020.6142868
CGTCGTA1800.020.55555510
CGATATG450.003825870620.55555516
GACGGAC3550.020.3239447
ATAACGA2050.019.85365712
CGAACGA2100.019.38095316
TACCGTC2050.018.9512187
CGCAAGA4250.018.7176482
CCGTCGT2100.018.5000029
AAGACGG4100.018.55
TAACGAA2200.018.513
GCGGGTA1103.851892E-818.523
GCGCAAG4150.018.277111
CGATAAC2250.018.08888810
TTATCTA1850.018.04
TCCGATA2300.017.6956528
AGACGGA4100.017.597566
GTTATCT2000.017.5749993
TACTTCG852.72322E-517.41176429
Produced by FastQC (version 0.11.5)