FastQCFastQC Report
Thu 28 Apr 2016
ERR1042425.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042425.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7627475
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT294290.3858288621070538No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT270400.35450788104844655No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT210690.2762250941497678No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100510.13177362102137338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCC12700.015.4409454
TAAACGC10300.015.26699128
GACGGAC15800.014.9873427
CGAACGA9800.014.7244916
ACGGACC16850.014.7121668
TCTAGCG9950.014.68844228
ACGAACG10400.014.23076915
GCGGGTA11050.014.23076823
TATACTG12400.014.1733885
TCTATAC9550.014.1413623
GTAAACG11200.014.04017827
CGCAAGA17400.013.928162
TACGACG11050.013.8959265
CAAGACG20000.013.7824994
CCGGTCG13700.013.77372420
TTAACGG4300.013.76744235
TCGTTTA13650.013.68864530
TACCGTC11100.013.6666677
CTAGCGG10750.013.59534829
AAGACGG18850.013.5437675
Produced by FastQC (version 0.11.5)