FastQCFastQC Report
Thu 28 Apr 2016
ERR1042424.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042424.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2515875
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT95570.379867839220947No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT86740.3447707060167933No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT40320.1602623341779699No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT32950.13096835097133205No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28480.11320117255428032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAAGA9400.018.3031922
ACGGACC9250.018.28
CAAGACG10600.017.8018884
CGGACCA9600.017.5364599
GACGGAC9500.017.5263167
TATTCCG650.001580640317.0769235
AAGACGG10100.017.0346555
TTCGCGG3850.016.81818232
GCAAGAC13750.016.0109083
TCGCGGG4450.015.79775233
AGACGGA10450.015.75598056
GCGCAAG11300.015.5530981
TAGGACA3000.015.4166674
CGAAAGC10850.015.17511619
GCGAAAG11000.014.96818318
AGCGAAA11000.014.96818317
ATTGACG5450.014.9357832
TGACCCG4200.014.53571413
ATACCGA1152.2117994E-514.4782616
TATCTAG6150.014.4390251
Produced by FastQC (version 0.11.5)