FastQCFastQC Report
Thu 28 Apr 2016
ERR1042422.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042422.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12743118
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT349370.2741636701472905No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT262400.20591506725434072No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT225520.17697395566767882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110750.025.624381
GTATCAA168350.016.8131872
ATAACGC19950.016.4135343
TAACGCC17250.015.979714
ACGGACC22550.015.5875848
GACGGAC23250.015.436567
TCGTTTA17850.014.61344630
CGCATCG18550.014.36118613
AATAACG19500.014.2307692
AACGCCG20000.013.9675015
CGGACCA25600.013.8027349
CGCAAGA27100.013.7896672
CCGGTCG19650.013.74554620
AAGACGG27750.013.7333345
TTAACGG7850.013.19745335
CGTTTAT19850.013.14105731
TATACTG26050.013.1381965
ATCGTTT20850.013.13189429
TCGCCGG21150.012.94562717
ATCGCCG21150.012.94562716
Produced by FastQC (version 0.11.5)