FastQCFastQC Report
Thu 28 Apr 2016
ERR1042420.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042420.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4264804
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT284560.6672287870673541No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT226590.531302259142507No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT211240.4953099837647873No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79750.18699569780932487No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC50230.11777797994937166No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA49910.1170276523844941No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117800.020.55731
ACGGACC4850.016.7835048
TAATACT8450.015.7633124
GTATTAG8400.015.1964281
GTATCAA171500.014.1204082
TAACGCC5400.014.0462974
GACGGAC5950.013.6806737
ACGCTGA17500.013.53142811
CGCATCG4650.013.52688113
TAGACCG2601.8189894E-1213.51923125
CGGACCA6100.013.3442629
TACGACG3750.013.325
TCGTTTA4650.013.12903230
AAGACGG6500.013.0923075
ACACGCT18100.013.0828729
GCGCAAG6650.013.0751881
CGCAAGA6800.013.0588242
ATAATAC6450.012.9069763
ACGAATA2602.3646862E-1112.8076933
ATAACGC5650.012.7699113
Produced by FastQC (version 0.11.5)