FastQCFastQC Report
Thu 28 Apr 2016
ERR1042419.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042419.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2705981
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC50180.18544106555071896No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG45790.1692177439531172No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT37080.13702978697928775No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC33680.12446502765540482No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA33670.12442807248092282No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT33210.12272813445475042No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA30580.11300892356598216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC957.1559043E-921.4210533
CTAGTAC1851.8189894E-1116.0000023
CGAACGA5750.015.44347916
TTAACGG1101.4532352E-515.13636435
TCTAGAC2750.014.83
ATAACGA6150.014.73983812
TAATACT4200.014.5357144
TACTCCG2550.014.5098045
TCGCTAA908.279119E-414.38888914
TAACGAA6450.014.05426413
TAAGACC4350.014.0344824
GGTTATC2405.456968E-1213.8749992
TCCGATA6600.013.7348488
TGTATCG950.00124583613.6315795
TACACGT950.00124583613.6315794
ACGGACC5300.013.6132088
CAAATCG6550.013.55725213
GTATTAG5900.013.483051
TCTAATA3050.013.3442622
ATACCGC5850.013.28205227
Produced by FastQC (version 0.11.5)