FastQCFastQC Report
Thu 28 Apr 2016
ERR1042418.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042418.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9642970
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT396990.4116885150529349No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT352090.3651260970427161No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT267360.27725897726530313No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122780.12732591722259842No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA98640.10229213613648076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC17900.016.432968
GACGGAC18650.015.7721197
AAGACGG20000.015.7249995
TCGTTTA13100.014.96946630
TTAACGG6050.014.67768635
GTATTAG21200.014.4858491
CGAGCCG18800.014.26861615
CGGACCA21300.013.7230049
TAACGCC16050.013.4859824
AGACGGA22350.013.4093976
TAATACT16950.013.3156354
TAACGGC6850.013.23357736
TATACTG14250.013.1122815
TATACCG4950.013.0808095
CAAGACG26450.012.7296784
CGTTTAT15600.012.68910331
CGAACGA16850.012.62611416
TAACGAA17100.012.54970713
TACCGTC12950.012.4285727
ATACCGC20000.012.302527
Produced by FastQC (version 0.11.5)