FastQCFastQC Report
Thu 28 Apr 2016
ERR1042417.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042417.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16282658
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT832730.5114214153487717No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT709330.4356352629896175No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT662160.40666579129771074No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254670.15640566791981997No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA227210.13954109949370674No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC207790.12761429982746061No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC174160.10696042378338967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA257800.023.1285881
TCGTTTA20950.015.36515530
GTATCAA407300.014.6301262
TATACTG23950.014.2129445
TTAGCGA9100.014.027472527
CGCATCG24800.013.4274213
CGAACGA34450.013.10304716
TAACGCC27100.013.0387454
TACGACG18000.012.955
TCTATAC16150.012.9442733
ATAACGC29700.012.7693613
GTATTAG33050.012.7624821
TAACGAA36650.012.51841813
ACACGCT70100.012.3773189
TAATACT33700.012.3516334
CGTTTAT26500.012.21698231
AACGCCG28200.012.20212655
ATAACGA37650.012.18592412
GATAACG38600.012.17357411
ATGGTCG23500.012.04468136
Produced by FastQC (version 0.11.5)